• AETDS, Uttarakhand, India.
  • ISO 9001:2015 Certified

Agricultural & Environmental Technology Development Society

(AETDS), U.S. NAGAR, UTTARAKHAND, INDIA.

Abstract

International Journal of Agricultural and Applied Sciences, June 2021, 2(1):130-133 

ISSN: 2582-8053

https://doi.org/10.52804/ijaas2021.2115

Short Communication

 

Assessment of In-vitro culture through Nodal explants of Dendrocalamus hamiltonii 

 

Abha Jha1,* and Sunila Das2

1HPS College Nirmali, BNMU Madhepura, India

2C. M. Sc. College Darbhanga, India

Corresponding author e-mail: abhajha202@gmail.com

(Received: 11/11/2020; Revised: 10/04/2021; Accepted: 15/06/2021)  

 

ABSTRACT

The present experimental study was aimed to overcome the traditional methods of propagation that limit the number of propagules by in-vitro regeneration through nodal explants of Dendrocalamus hamiltonii with a comparative study of growth regulators during the shooting and rooting process. Dendrocalamus hamiltonii is distributed from the Himalayas (Nepal) to the northern part of Burma. Collection of explants was done from different selected sites of CPTs. There was the use of HgCl2 and Ca (OCl)2 as sterilizing agents in different concentrations and its effect was visualized during the sprouting stage. Culm explants were cultured in a bottle containing White media (Wm) supplemented with BA and Kinetin for sprouting and IAA, IBA, NAA for rooting. There is also the use of IAA+IBA+NAA in combined form as a supplementary solution 0.1% HgCl2 treatment for 20-minute results into77.80% aseptic buds and 72% bud -break. Among the used growth-hormones, BA with concentration 0.25mg/l and 0.50mg/l respectively were appropriate for shoot-multiplication rate, 4.01±0.3 and 4.3±0.4 were ideal observation incorporation with BA (1.00mg/l) and BA (1.50mg/l) respectively. Maximum sprouting rate14.77±3.37with application of BA (2.00mg/l) and maximum shoot length4.3±0.4 is observed at BA (1.50mg/l). The applications of rooting hormone IAA+IBA+NAA in the concentration of 1.0 mg/l results in 72.5±0.3(rooting) and 11.1±0.3 (av. No. of the root).

Keywords: CPTs, HgCl2, sterilizing agents, BA, Kinetin, sprouting rate